Application

Research CategorySignaling

Western blot 1:500 - 1:1000, with detection by 125I-labeled anti-IgG.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Research Sub CategoryGPCR, cAMP/cGMP & Calcium Signaling

Anti-G Protein Giα-2 Antibody, clone L5 is an antibody against G Protein Giα-2 for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Recombinant Gialpha-2, purified by the method of Linder (1990).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin, in 0.02M phosphate buffer, 0.25M sodium chloride, pH 7.6, with 0.1% sodium azide.

Format: Purified

Specificity

Gialpha-2. Strong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.

Storage and Stability

Maintain at 2-8°C.